A549 Lysate

Size

£363.00

SKU:: 551811
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Extra Details:: A549 cells are adenocarcinomic human alveolar basal epithelial cells. The A549 cell line was initiated from the removal and culturing of cancerous lung tissue in the explanted tumor from 58-year-old male, in 1972. A549 cells is able to synthesize lecithin and contain high level of unsaturated fatty acids, which are important to maintain the membrane phospholipids in cells. A549 cell line are widely used as an in vitro model for a type II pulmonary epithelial cell model for drug metabolism and as a transfection host. Quality Control: Western Blot analyzed by both GAPDH and Beta tubulin antibody Preparation Before Usage: Heat lysate to 95°C for 5 minutes and rapidly cool before use. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.