A431 Lysate

Size

£363.00

SKU:: 551810
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Extra Details:: A431 cells were established from an epidermoid carcinoma in the skin/epidermis of an 85 year old female patient. A431 cells are used in studies of the cell cycle and cancer-associated cell signalling pathways since they express abnormally high levels of the EGFR. As such they are often used as a positive control for EGFR expression. Quality Control: Western Blot analyzed by both GAPDH and Beta tubulin antibody Preparation Before Usage: Heat lysate to 95°C for 5 minutes and rapidly cool before use. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.