551599
PsuI
Size
£377.00
- SKU:
- 551599
- Storage Conditions:
- -70°C
- Supplier:
- United States Biological
- Extra Details:
- 5'R↓G ATCY3 '3'YCTAG↑R5' PsuI restriction enzyme recognizes R^GATCY site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: BstX2I, BstYI, MflI, XhoII. PsuI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers. Thermal Inactivation: Incubation at 80°C for 5 min. Applications • Molecular cloning • Restriction site mapping • Genotyping • Southern Blot • Restriction fragment length polymorphism (RFLP) • SNP analysis Note: The Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended. Methylation Sensitivity: Not dam methylation-sensitive, Not dcm methylation-sensitive, Not CpG methylation-sensitive Enzyme: PsuI Compatible Buffer: 10x FastDigest Buffer/FastDigest Green Buffer Sensitive to Heat Inactivation: No Optimal Reaction Temperature: 37°C Type IIS RE: No Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Dry Ice