551234
CD74 (aa159-171)
Size
£642.00
- SKU:
- 551234
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Synthetic peptide corresponding to aa159-171, C-KGSFPENLRHLKN, from human CD74 at the internal region.
- Clone:
- Search Type in product code or name: Goat Anti-CD74 (aa159-171) Antibody (EB12551) Related Products: EBP12551 - Immunizing Peptide, EBP12552 - Immunizing Peptide, Goat Anti-CD74 Antibody Code Name Applications Availability Size Tested species Price Grade EB12551 Goat Anti-CD74 (aa159-171) Antibody Pep-ELISA, WB, IHC 100µg specific antibody in 200µl Human $324.00 Ordering Information for United States of America (change) Order direct from Everest $49.00: Overnight shipping (not applicable to SB-shifix) to United States of America Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC. Order offline OR Order from our local distributor ARP American Research Products, Inc View contact details Product details Reviews Protocols and MSDS EB12551 (0.01µg/ml) staining of Human Tonsil lysate (35µg protein in RIPA buffer). Detected by chemiluminescence. EB12551 (5µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining. Click here to download PDF datasheet. Target Protein Principal Names: CD74, DHLAG, HLADG, Ia-GAMMA, CD74 molecule, major histocompatibility complex, class II invariant chain, CD74 antigen, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated), HLA-DR-gamma, Ia-associated invariant chain, MHC HLA-DR gamma chain, gamma chain of class II antigens Official Symbol: CD74 Accession Number(s): NP_001020330.1; NP_004346.1 Human GeneID(s): 972 Important Comments: This antibody is expected to recognize isoform a (NP_001020330.1) and isoform b (NP_004346.1). Immunogen Peptide with sequence C-KGSFPENLRHLKN, from the internal region of the protein sequence according to NP_001020330.1; NP_004346.1. Please note the peptide is available for sale. Purification and Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. Applications Tested Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 33kDa band observed in Human Tonsil lysates (calculated MW of 33.5kDa according to NP_001020330.1). Recommended concentration: 0.01-0.03µg/ml. Primary incubation was 1 hour. IHC: Paraffin embedded Human Spleen. Recommended concentration: 5µg/ml. Species Reactivity Tested: Human Expected from sequence similarity: Human
- Format:
- Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
- Extra Details:
- Applications: Suitable for use in Peptide ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Peptide ELISA: 1:128,000 Western Blot: 0.01-0.03ug/ml. Immunohistochemistry: (paraffin) 5ug/ml. Heat induced antigen retrieval in citrate buffer, pH 6.0 is required. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice