551140
Actin, beta
Size
£895.00
- SKU:
- 551140
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
- Clone:
- AC-74
- Format:
- Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
- Extra Details:
- Beta-actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western Blot. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 0.5-1ug/ml. using total cell extracts of human or chicken fibroblasts Immunocytochemistry: 10-40ug/ml using human or chicken fibroblasts Optimal dilutions to be determined by the researcher. Positive Control: Cultured human or chicken fibroblasts Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice