Tryptase (Biotin)

Size

£518.00

SKU:: 550431
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Gly161-Tyr262 from human Tryptase, expressed in E.coli.
Clone:: C16
Format:: Supplied as a liquid in 0.01M PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Extra Details:: Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 0.5-2ug/ml Immunocytochemistry (formalin fixed cells): 5-20ug/ml Immunohistochemistry (formalin fixed frozen): 5-20ug/ml Immunohistochemistry (paraffin): 5-20ug/ml ELISA: 0.05-2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.