RPL-αMan, Recombinant, Pseudomonas aeruginosa, Hexa-His-Tag

Size

£718.00

SKU:: 544495
Molecular Weight:: 13130
Storage Conditions:: -70°C
Supplier:: United States Biological
Extra Details:: Recombinant lectin protein with a Hexa-His Tag from Pseudomonas aeruginosa αMan, expressed in E. coli. Purity: ≥95% (SDS PAGE analysis using Coomassie Blue detection and gel Permeation Chromatography) Form: Supplied as a liquid in PBS, pH 7.2. Specificity: Fucose/Mannose: Lewis a (Lea), Lewis x (Lex) & terminal α-mannose Molecular Weight: 13,130D (Monomer), Protein is a dimer under physiological conditions (PBS, pH 7.2) Usage Guidelines: •RPL activity requires metal ions. All buffers must be supplemented with 1mM CaCl2, MgCl2 and MnCl2. •Recommended dilution to desired working concenration (≤20ug/ml) in TBS, pH 7.6. •Following dilution with TBS and supplemented with metal ions, the RPL should be incubated for a minimum of 30 minutes prior to use to allow the metal complexation. Theoretical Ext. Coefficient [E0.1%]: 0.532 Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.