544411
TIM-3 (PE)
Size
£678.00
- SKU:
- 544411
- Purity:
- Purified by Protein A affinity chromatography from HEK293 supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Human
- Immunogen:
- Recombinant protein corresponding to human TIM-3, expressed in HEK293 cells.
- Clone:
- 20E16
- Format:
- Supplied as a liquid in PBS, pH 7.4, 20% glycerol. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- T-cell immunoglobulin mucin 3, a transmembrane glycoprotein and member of the immunoglobulin superfamily, is preferentially expressed by differentiated CD4+ Th1 cells and CD8+ Tc1 (cytotoxic) cells, but not by CD4+ Th2 cells or CD8+ Tc2 cells. Tim-3 acts as a negative regulator of Th1-mediated inflammatory diseases including GVHD, type I diabetes, and EAE (experimental autoimmune encephamyelitis), promotes immunological tolerance, and is a receptor for galectin-9, shown to induce cell death of Th1 cells in vitro. Tim-3 is also involved in the regulation of macrophage activation, acts as a phagocytic receptor, and has been shown to recognize apoptotic cells via the IgV domain. Studies have shown Tim-3 significantly inhibits the efficient phagocytosis of apoptotic cells by PEMs (peritoneal exudate Mac1+ cells) ( Nakayama et al. 2009). Applications: Suitable for use Flow Cytometry and Immunofluorescence. Optimal dilutions to be determined by the researcher. Recommended Dilution: Flow Cytometry: incubate cells with 20ug/ml of PE-anti-TIM-3 antibody in 100ul of FACS buffer for 20 min at 4°C in dark. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice