TIGIT, Recombinant, Human, aa22-141, Fc-Tag, Avi-Tag (Biotin)

Size

£1,015.00

SKU:: 544408
Molecular Weight:: 41.6
Purity:: ≥90%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 20% glycerol. Labeled with Biotin.
Extra Details:: Source: Recombinant protein corresponding to aa22-141 from human TIGIT, fused to Fc region of human IgG1 and C-terminal Avi-Tag, expressed in a HEK293 cell. Molecular Weight: ~41.6kD Applications: Suitable for the use in study of protein binding and for screening small molecules and antibodies. Other applications not tested. Assay Conditions: 100nM of biotinylated TIGIT was incubated for 1 hour with the indicated concentration of CD155 in a 10ul reaction in with TIGIT Assay Buffer. Chelate Acceptor beads were added and incubated for 30min. followed by Strep-donor beads for 30min and alpha counts were read. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.