PD-L1, Recombinant, Human, Fc-Tag

Size

£887.00

SKU:: 544371
Molecular Weight:: 53
Purity:: ≥90%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 20% glycerol.
Extra Details:: Source: Recombinant protein corresponding to aa19-239 from human PD-L1, fused at the C-terminus with the Fc portion of mouse IgG2a, expressed in a HEK293 cells. Molecular Weight: ~53kD Applications: Suitable for the use in study of protein-protein interaction and in vitro and in vivo protein function. Other applications not tested. Assay Conditions: Add 50ul of PD-1-Biotin in assay buffer (PBS with 0.1% BSA) to the wells precoated with PD-L1 on a white maxisorp plate. Incubate the reaction mixture at room temperature for 2 hours. Wash and block wells. Then add streptavidin-HRP and incubate for 1 hour. Finally, add HRP chemiluminescent substrates and read luminescence. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.