PD-L1 (CD274), Recombinant, Human, aa19-239, Fc-Tag

Size

£734.00

SKU:: 544364
Molecular Weight:: 52
Purity:: ≥90%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 20% glycerol.
Extra Details:: Recombinant protein corresponding to aa19-239 from human PD-L1, fused at the C-terminus to the Fc portion of human IgG1, expressed in HEK293 cells. Molecular Weight: ~52kD Assay Conditions: PD-L1 binding to PD-1 was tested following protocol. Briefly, wells were coated with 10ng of PD-L1 in PD-1 BioAssay™ buffer. Varying concentrations of biotinylated PD-1 was added and wells were incubated at room temp for 2 hours before adding strep-HRP, followed by chemiluminescent substrate. Luminescence was read on a chemiluminescent plate reader. Applications: Suitable for use in the study of protein-protein interaction and in vitro and in vivo protein function. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.