PD-L1 (Biotin)

Size

£940.00

SKU:: 544362
Purity:: Purified by Protein A affinity chromatography from HEK293 supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Human
Immunogen:: Recombinant protein corresponding to human PD-L1, fused to Avi-Tag at N-terminal, expressed in HEK293. AA Sequence: Heavy Chain: aa1-210 Light Chain: aa1-214(end)
Clone:: 20E12
Format:: Supplied as a liquid in PBS, pH 7.4, 20% glycerol. Labeled with Biotin.
Extra Details:: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PDCD-1 that mediate inhibitory signals from the APC. PDCD-1 can bind two different but related ligands, PDL-1 and PDL-2. PDL-1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells. This suggests that PDL-1 may be useful in reducing allogenic CD4+ memory T-cell responses to endothelial cells, thereby reducing the likelihood of host immune responses to allografts. At least two isoforms of PDL-1 are known to exist; this antibody is specific to the larger isoform. PDL-1 antibody has no cross-reactivity to PDL-2. Applications: Suitable for use as an antagonist to block PD-L1 signaling and binding to PD-1 in biochemical assays. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Assay Conditions: Anti-PD-L1-Biotin was titrated from 0-50nM with a 2-fold dilution series in presence of 100nM PD-L1-FLAG and incubated for 1 hour at RT. Binding was detected with Anti-FLAG-Eu and Streptavidin-APC. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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