PD-1, Recombinant, Human, aa25-167, FLAG-Avi-His-Tag

Size

£646.00

SKU:: 544353
Molecular Weight:: 20
Purity:: ≥90%
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 20% glycerol.
Extra Details:: Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen-specific manner. Possible cell death inducer, in association with other factors. Source: Recombinant protein corresponding to aa25-167 from human PD-1, fused to FLAG-Avi-His-Tag at C-terminal, expressed in HEK293 cells. Molecular Weights: ~20kD Applications: Suitable for use in study of protein binding and screening small molecular inhibotors. Other applications not tested. Assay Conditions: 10nM PD-L1, Biotin labeled was mixed with varying amounts of PD-1, FLAG-Tag in PD-1 Assay Buffer for 1hr at RT. FLAG-acceptor beads (left) or Nickel-acceptor beads (right) were added and incubated for 30min at RT followed by streptavidin-conjugated donor beads for 30min at RT. Alpha counts were read. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.