FGL1, Recombinant, aa23-312, Fc-Tag, Avi-Tag (Biotin)

Size

£1,015.00

SKU:: 544287
Molecular Weight:: 63
Purity:: ≥90%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 20% glycerol. Labeled with Biotin.
Extra Details:: Source: Recombinant protein corresponding to aa23-312 from human FGL1, fused at the C terminus to the Fc portion of human IgG1 and with a C-terminal Avi-Tag, expressed in HEK293 cells. Molecular Weight: ~63kD Assay Conditions: FGL1-biotin was titrated from 0 to 160nM with a 2-fold dilution series in presence of 0 and 400ng/well of immobilized LAG3-His and incubated for 1 hour at RT. Binding was detected with Streptavidin-HRP and ELISA ECL Substrate. Applications: Suitable for use in the study of protein binding and screening small molecules. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.