CRTAM, Recombinant, Human, aa18-286, Fc-Tag, Avi-Tag

Size

£633.00

SKU:: 544266
Application:: WB
Molecular Weight:: 25
Purity:: ≥90%
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 20% glycerol.
Extra Details:: Source: Recombinant protein corresponding to aa18-286 from human CRTA, fused to C-terminal Avi-Tag fused to the Fc region of Human IgG1, expressed in HEK293 cells. Applications: Suitable for use as a positive control for Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Assay Conditions: Anti-PD-L1-Biotin was titrated from 0-50nM with a 2-fold dilution series in presence of 100 nM PD-L1-FLAG and incubated for 1 hour at RT. Binding was detected with Anti-FLAG-Eu and Streptavidin APC. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.