CD40, Recombinant, Human, aa21-193, Fc-Tag, Avi-Tag (Biotin)

Size

£1,015.00

SKU:: 544235
Molecular Weight:: 48
Purity:: ≥90%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 20% glycerol. Labeled with Biotin.
Extra Details:: Source: Recombinant protein corresponding to aa21-193 from human CD40, fused at the C-terminus with the Fc portion of Human IgG1 and with a C-terminal Avi-Tag, expressed in a HEK293 cells Molecular Weight: ~48kD Applications: Suitable for the use in study of western blots, studying protein binding, and for screening small molecules and antibodies. Other applications not tested. Assay Conditions: Reaction done according to standard ELISA BioAssay™ kit protocol. Buffer is PBS + 0.1% BSA. Blocking buffer is Superblock + 0.05% Tween-20. Plate was coated with 100ng/well CD40L at 4°C overnight. Various concentrations of biotinylated CD40 (0-1uM) added for 2h at RT. Strep-HRP added for 1h at RT. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.