CD38, Recombinant, Human, aa43-300, His-Tag

Size

£894.00

SKU:: 544230
Molecular Weight:: 31
Purity:: ≥90%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 20% glycerol.
Extra Details:: CD38, also known as ADP-ribosyl cyclase, is a Type II integral membrane protein. The enzyme is able to transform NAD(P)+ into three different products with calcium mobilizing ability; cyclic ADP-ribose, NAADP+ , and ADP-ribose 3. CD38 is expressed in B and T lymphocytes, osteoclasts, and in cardiac, pancreatic, liver and kidney cells 1, 4. Through its production of cyclic ADP-ribose, CD38 modulates calcium-mediated signal transduction in many types of cells, including neutrophils and pancreatic β cells 5, 6. Source: Recombinant protein corresponding to aa43-300 from human CD38, fused to His-Tag at C-terminal, expressed in a HEK293 cells. Molecular Weight: ~31kD Applications: Suitable for the use in study of protein binding and for screening small molecules and antibodies. Other applications not tested. Assay Conditions: Add CD38 enzyme to 50ul reaction mix containing NGD+ (Nicotinamide Guanine Dinucleotide). Incubate 4 min at room temperature. Read fluorescence (delta exc= 300nm, delta em= 410nm). Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Dry Ice