CD20 Agonist

Size

£964.00

SKU:: 544209
Purity:: Purified by Protein A affinity chromatography from HEK293 supernatant.
Storage Conditions:: -70°C
Supplier:: United States Biological
Host:: Human
Immunogen:: Recombinant protein corresponding to aa142-184 from human CD20 Agonist, expressed in HEK293 cell.
Clone:: 20E2
Format:: Supplied as a liquid in PBS, pH 7.4, 20% glycerol.
Extra Details:: CD20 (also known as membrane spanning 4-domains A1 or MS4A1) is a glycosylated phosphoprotein expressed on the cell surface of B cells. Although the functional significance of CD20 is not clear, it has been shown to regulate intracellular calcium levels. CD20 is a highly attractive target antigen for immunotherapy because it is expressed in more than 90% of patients with B-cell lymphoma. First approved in 1997, Rituximab (Rituxan) is a chimeric monoclonal antibody targeting CD20 and has been classified by the World Health Organization as an “Essential Medicine”. Since then, additional monoclonal antibodies against CD20 have been approved or are being tested in clinical trials for the treatment of multiple sclerosis (MS), chronic lymphocytic leukemia (CLL), follicular lymphoma, diffuse large B cell lymphoma (DLBCL), rheumatoid arthritis, non-Hodgkin’s lymphoma, systemic lupus erythematosus, and myalgic encephalomyelitis (chronic fatigue syndrome). More recently, anti-CD20-CD19 bispecific CAR-T cells have been developed. Features the constant region of the human IgG1 isotype and the mouse variable region of rituximab (amino acids 142-184). This antibody is cytotoxic to WIL2-S cells in the presence of the rabbit complement system. Rituximab is an anti-CD20 chimeric recombinant monoclonal antibody used for research of certain autoimmune diseases and cancer. Applications: Suitable for use in detection of CD20 and binding to B cells or other cells expressing CD20 on their surface. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Assay Conditions: WIL2-S cells were treated with anti-CD20 + 10% rabbit complement. Cell viability was determined using AlamarBlue Indicator. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Dry Ice