B7-1(CD80), Recombinant, Mouse, aa38-246, Fc-Tag, Avi-Tag (Biotin)

Size

£1,015.00

SKU:: 544163
Molecular Weight:: 52
Purity:: ≥90%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 20% glycerol. Labeled with Biotin.
Extra Details:: Source: Recombinant protein corresponding to aa38-246 from mouse B7-1, fused at the C-terminus of the Fc portion of human IgG1 and with a C-terminal Avi-Tag, expressed in HEK293 cells. Molecular Weight: ~52kD Assay Conditions: Reaction done similar to CTLA4:B7-1[biotin] BioAssay™ kit protocol. Buffer is PBS + 0.1% BSA. Blocking buffer is Superblock + 0.05% Tween-20. Coat protein (mouse CTLA4) was added overnight at 4 deg 100ng/well. Various amounts of biotinylated ligand (mouse B7-1[B]) (0-50ng) added for 2h at RT. Strep-HRP added for 1h at RT. Applications: Suitable for use in the study of protein-protein interaction and in vitro and in vivo protein function studies. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.