B7-1, Recombinant, Human, aa35-242, Fc-Tag (Biotin)

Size

£804.00

SKU:: 544161
Molecular Weight:: 53
Purity:: ≥90%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 20% glycerol. Labeled with Biotin.
Extra Details:: Source: Recombinant protein corresponding to aa35-242 from human B7-1, fused at the C-terminus to the Fc portion of human IgG1 and with a C-terminal Avi-Tag, expressed in HEK293 cells. Molecular Weight: ~53kD Assay Conditions: Reaction done according to CTLA4:B7-1[biotin] BioAssay™ kit protocol. Buffer is PBS + 0.1% BSA. Blocking buffer is Superblock + 0.05% Tween-20. Coat protein was added ON at 4 deg 100ng/well. Various concentrations of biotinylated ligand (0-8ng/ul, 0.5ng/ul is the recommended kit condition) added for 2h at RT. Strep-HRP added for 1h at RT. Applications: Suitable for use in the study of protein-protein interaction and in vitro and in vivo protein function studies. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.