ACE2, Recombinant, Human, His-Tag, Avi-Tag

Size

£709.00

SKU:: 544149
Purity:: ≥95% (SDS-PAGE). Endotoxin: <1EU/ug (LAL)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from a 0.22um filtered solution of PBS, pH 7.4. Reconstitute to 0.1-0.5mg/ml in sterile ddH2O.
Extra Details:: ACE2, also known as angiotensin-converting enzyme 2, is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE. ACE2 cleaves angiotensin 1 to angiotensin 1-9 and angiotensin 2 to angiotensin 1-7 as a carboxypeptidase. It may be an important regulator of heart function and have a protective role in acute lung injury. Recombinant protein corresponding to Gln18-Ser740 from human ACE2, fused to His-Tag and Avi-Tag at C-terminal, expressed in HEK293 cells. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2-His at 2ug/mL (100ul/well) can bind Recombinant 2019-nCoV Spike S1 with a linear range of 1.5-104.5ng/ml. Storage and Stability: Lyophilized and reconstituted products may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.