2019-nCoV Spike S1, Recombinant, His-Tag

Size

£576.00

SKU:: 544145
Purity:: ≥90% (SDS-PAGE). Endotoxin: <1EU/ug (LAL)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from a 0.22um filtered solution of PBS, pH 7.4. Reconstitute to 0.1-0.5mg/ml in sterile ddH2O.
Extra Details:: The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. Recombinant protein corresponding to Val16-Arg685 form 2019-nCoV Spike S1, fused to His-Tag at C-terminal, expressed in HEK293 cells. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Recombinant 2019-nCoV Spike S1 at 2ug/mL (100ul/well) can bind Recombinant Human ACE2 with a linear range of 0.5-8.7ng/ml Storage and Stability: Lyophilized and reconstituted products may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.