2019-nCoV, S1+S2 ECD (S-ECD), Recombinant, His-Tag

Size

£869.00

SKU:: 544133
Purity:: >97% (SDS-PAGE). Endotoxin: <0.1EU/ug (LAL)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4.
Extra Details:: The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. Recombinant protein corresponding to Val11-Gln1208 from 2019-nCoV S1+S2 ECD (S-ECD), fused to His-Tag at C-terminal, expressed in HEK293 cells. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Recombinant 2019-nCoV Spike S1+S2 ECD-His at 2ug/ml (100ul/well) can bind Recombinant Human ACE2 with a linear range of 1.5-115.4ng/ml. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.