Canine Parvovirus

Size

£730.00

SKU:: 544055
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Canine parvovirus.
Clone:: 8H7
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Extra Details:: Canine parvovirus (CPV) belongs to the genus Protoparvovirus and the family Parvoviridae and causes a highly contagious and fatal disease in canines (Kelly, 1978). The symptoms include lethargy, loss of appetite, fever, vomiting, and severe (often bloody) diarrhea. Parvoviral infection must be considered as a possible diagnosis in any young canine with vomiting and/or diarrhea. Young and adult canines usually become infected when they ingest a virus that has been passed in the feces of an infected canine. Vomiting and diarrhea can cause rapid dehydration, and most deaths from parvovirus occur within 48 to 72 hours following onset of clinical signs. CPV is a non-enveloped DNA virus with an ~5000nt, single-stranded DNA genome containing two open reading frames (ORFs). The first ORF encodes two non-structural proteins, NS1 and NS2. The second ORF encodes two structural proteins, VP1 and VP2. VP1 and VP2 each encode parts of the viral capsid, which is assembled from 54 copies of VP2 and 6 copies of VP1. VP2, the major capsid protein, is also the major antigenic protein and determines viral tissue tropism and host range. The disease can be prevented by vaccination and CPV‑2 vaccine is recommended as a core vaccine that should be given to all canines. However, there are concerns regarding the complete efficacy of existing vaccines against antigenic variants which may evolve to escape the immune system via antigenic drift, resulting in vaccine failure. (Zhou et al., 2017). Applications: Suitable for use in Immunodiffusion, Hemagglutinin Inhibition, ELISA and Lateral Flow. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice