Human Immunodeficiency Virus (HIV-1) gp120

Size

£659.00

SKU:: 544040
Purity:: Purified by Protein A affinity chromatography from ascites.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Clone:: 1996
Format:: Supplied as a liquid in 0.01M PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: The human immunodeficiency virus HIV-1 establishes persistent infections in humans which lead to acquired immunodeficiency syndrome (AIDS). The HIV-1 envelope glycoproteins gp120 and gp41 are assembled into a trimeric complex which allows virus entry into target cells. Gp120 is a glycoprotein on the HIV envelope and acts as a receptor on the surface of the viral particle, binding to CD4 and secondary receptors on macrophages and T lymphocytes. The viral spike of HIV-1 is composed of three gp120 envelope glycoproteins attached noncovalently to three gp41 transmembrane molecules. Viral entry is initiated by binding to the CD4 receptor on the cell surface, which induces large conformational changes in gp120. This starts the process by which HIV fuses its viral membrane with the host cell membrane and enters the host cell. Gp120 has been shown to interfere with the normal function of CD4 and is at least partly responsible for the cytopathic effect of HIV (Pancera et al., 2010). HIV-1 continues to be a major global health threat and the development of an effective vaccine is highly desirable to eradicate HIV/AIDS. Most existing viral vaccines work through the induction of neutralizing antibodies (NAbs) that block infection and/or viremia (Plotkin, 2010). However, despite great efforts, to date no HIV-1 vaccine has been developed that elicits broadly neutralizing antibodies (bNabs) to protect against infection. High antigenic diversity and densely populated N-linked glycan sites, which cover nearly the entire HIV-1 envelope protein (Env), are major roadblocks for the development of bNAbs by vaccination (van Schooten and van Gils, 2018). GP120 has been a long running target for HIV vaccine research (Duarte, 2018) but its chemical and structural properties have made it difficult for antibodies to bind to it. However, primate studies have demonstrated that recombinant gp120 can elicit protective immunity against a homologous strain of HIV-1 (Berman et al., 1990). Applications: Suitable for use in ELISA and Immunofluoresence. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:10-1:50 ELISA: 1:20-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice