542653
Stat3, phosphorylated (Ser727)
Size
£488.00
- SKU:
- 542653
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide derived from human Stat3 around the phosphorylation site of S727.
- Format:
- Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Signal transducer and activator of transcription 3 encoded by STAT3 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. Signal transducer and activator of transcription 3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. Signal transducer and activator of transcription 3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in STAT3 are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Applications: Suitable for use in Western Blot, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:2000 Immunofluorescence: 1:50-1:200 Immunohistochemistry (paraffin): 1:100-1:300 Immunoprecipitation (lysate): 2-5ug/mg ELISA: 1:10,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice