OPN

Size

£371.00

SKU:: 540293
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to aa230-310 of human OPN at C-terminal.
Format:: Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Extra Details:: Secreted phosphoprotein 1 encoded by SPP1 is involved in the attachment of osteoclasts to the mineralized bone matrix. Secreted phosphoprotein 1 is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to secreted phosphoprotein 1. Secreted phosphoprotein 1 is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for SPP1. Applications: Suitable for use in Western Blot, Immunohistochemistry, and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:2000 Immunohistochemistry (paraffin): 1:100-1:300 ELISA: 1:20,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.