Myosin Heavy Chain

Size

£371.00

SKU:: 539367
Application:: IF
Purity:: Purified by immunoaffinity chromatography from ascites.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to Myosin Heavy Chain.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Skeletal muscle Myosin or myosin II is the motor protein that generates force to drive muscle contraction. It is a 520kD hexamer comprised of two heavy chains and four light chains. Myosin heavy chain is 220kD in size and consists of a long coiled-coil domain tail that mediates dimerization of the two heavy chains and a globular head region that mediates ATP-dependent sliding of actin filaments. Myosin heavy chain can be proteolytically cleaved to produce heavy meromyosin, which includes the S1 motor domain (head region) and first third of the coiled coil domain, and light meromyosin, which includes the C-terminal two thirds of the coiled coil domain. Applications: Suitable for use in Immunofluorescence. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.