HA-Tag (HRP)

Size

£585.00

SKU:: 537083
Application:: WB
Purity:: Purified by immunoaffinity chromatography from ascites.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to aa98-106 of HA-Tag.
Clone:: 4F6
Format:: Supplied as a liquid in PBS, pH 7.4, 50% glycerol. Lableled with horseradish peroxidase (HRP).
Extra Details:: Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA-Tag is derived from the HA-molecule corresponding to aa98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA-Tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:3000 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice