535745
E-cadherin
Size
£488.00
- SKU:
- 535745
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to aa810-890 of human E-cadherin at C-terminal.
- Format:
- Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- CDH1 encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in CDH1 are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. CDH1 is present in a gene cluster with other members of the cadherin family on chromosome 16. Applications: Suitable for use in Western Blot, Immunofluorescence, Immunohistochemistry, and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:2000 Immunofluorescence: 1:200-1:1000 Immunohistochemistry (paraffin): 1:100-1:300 ELISA: 1:20,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice