Canine Heartworm

Size

£639.00

SKU:: 532406
Purity:: Purified by Protein A affinity chromatography from ascites.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Clone:: 3233
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: Dirofilaria immitis is a parasitic nematode responsible of canine and feline cardiopulmonary dirofilariasis in both domestic and wild hosts, and the causal agent of human pulmonary dirofilariasis. It is a zoonotic parasitic disease transmitted by mosquitoes and mainly located in temperate, tropical, and subtropical areas of the world (Simón et al., 2009b). In the United States, canine dirofilariasis has been reported from all states, and D. tenuis in raccoons is common in many areas where raccoons are found. Canine and human dirofilariasis are most prevalent in eastern and southeastern states, although rates are increasing rapidly in a number of western states. D. immitis is the Dirofilaria species most commonly reported to cause dirofilariasis in humans in the United States. In Europe, Dirofilaria immitis is distributed mainly in the Mediterranean region, including Italy, Greece and Spain, whereas in northern European countries, D. repens is the Dirofilaria species most commonly reported to cause dirofilariasis among humans ((CDC, 2019; Miterpáková et al., 2018). Different species of culicid mosquitoes (Culex spp., Aedes spp., Anopheles spp.) act as an intermediate stage in order to complete their life cycle. In a canine host, sexually mature worms produce microfilariae that circulate in the blood and are ingested by mosquitoes during a blood meal. In mosquitoes, the microfilariae develop into larvae that migrate to the proboscis where they are ready to infect another host during a blood meal. Heartworms typically live for 3–5 years in an animal’s body. At least 70 species of mosquitoes can transmit heartworm disease, including Aedes, Anopheles, Culex and Mansonia (Atkins, 2019). Diagnosis of heartworm infection relies upon detecting antigen of Dirofilaria immitis in serum, plasma, or whole blood samples. ELISA is considered the most sensitive platform and detects antigen in as many as 85.7% of canine infected with a single adult female worm; reported sensitivity for microtiter plate ELISA when at least 3 adult female worms are present is 100% (McCall, 2001). Many of the lateral flow immunochromatographic assays and the membrane-bound ELISAs, which are designed to provide rapid in-clinic results, also demonstrate good sensitivity and can detect antigen in 46–76.2% of patients infected with a single female worm and 84–100% of patients with 3 or more female worms [McCall, 2001; Courtney and Zeng, 2001; Atkins, 2003). Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 1:20-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice