Canine Heartworm

Size

£639.00

SKU:: 532405
Purity:: Purified by Protein A affinity chromatography from ascites.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Clone:: 3232
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: Dirofilaria immitis causes a severe disease (heartworm disease) in canine and other carnivores and occasionally infects humans, while D. repens usually causes a non-pathogenic subcutaneous infection in canine and it is the principal agent of human dirofilariosis in the Old World (Pampiglione and Rivasi, 2000; Capelli et al., 2018). In Europe, Dirofilaria immitis is distributed mainly in the Mediterranean region, including Italy, Greece and Spain, whereas in northern European countries, the D. repens species is dominant (Miterpáková et al., 2018). The prevalence of the disease is influenced by climate and topography, but most importantly, by the presence of mosquito vectors. Different species of culicid mosquitoes (Culex spp., Aedes spp., Anopheles spp.) act as an intermediate stage in order to complete the worms life cycle. When taking a blood meal from a microfilaremic host, the mosquitoes become infected and the microfilariae develop to the third-stage larvae (L3) in the malpighian tubules of the mosquitoes (Cancrini and Kramer, 2001), which are deposited on the host while the mosquito is taking a blood meal, becoming sexually mature within a few months in the main pulmonary arteries and right ventricle (Morchón et al., 2012). D. immitis adult worms can cause pulmonary artery blockage in canine, leading to an illness that can include cough, exhaustion upon exercise, fainting, coughing up blood, and severe weight loss. Heartworm infection may result in serious complications for the host, typically culminating in the host’s death, most often as the result of secondary congestive heart failure. There is no vaccine and medications are based on macrolide prophylaxis which are generally safe and effective at preventing infection. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 1:20-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice