Thioredoxin-1, Recombinant, Mouse, aa1-105, His-Tag

Size

£544.00

SKU:: 532384
Molecular Weight:: 14.1
Purity:: ≥90% by SDS-PAGE under reducing conditions and visualized by coomassie blue stain. Endotoxin: ≤1EU/ug (LAL).
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.
Extra Details:: Thioredoxin-1 is a low molecular weight redox protein. Thioredoxin-1 contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. It is involved in the first unique step in DNA synthesis. Thioredoxin-1 also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation. Recombinant protein corresponding to aa1-105 from mouse Thioredoxin-1 alpha, fused to His-Tag, expressed in E. coli. Accession: NP_035790. Molecular Weight: ~14.1kD (128aa) confirmed by MALDI-TOF Biological Activity: Specific activity is >60 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650nm resulting from the reduction of insulin Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMVKLIES KEAFQEALAA AGDKLVVVDF SATWCGPCKM IKPFFHSLCD KYSNVVFLEV DVDDCQDVAA DCEVKCMPTF QFYKKGQKVG EFSGANKEKL EASITEYA Applications: Suitable for use in Enzyme Activity and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice