PIN1

Size

£665.00

SKU:: 526978
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to human PIN1.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Pin1, a member of the parvulin family of peptidyl-prolyl isomerases (PPIase), has been implicated in the G2/M transition of the mammalian cell cycle (1-6). Pin1 is a small (18kD) protein with two distinct functional domains: an N-terminal WW domain and a C-terminal PPlase domain. Pin1 interacts with several mitotic phosphoproteins, including Plk1, cdc25C and cdc27, and is thought to act as a phosphorylation-dependent PPlase for these target molecules (7-9). Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight. Immunohistochemistry: 1:50-1:200 Immunofluorescence (IF-IC): 1:100-1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice