PICK1

Size

£665.00

SKU:: 526945
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to human PICK1. Species sequence homology: Porcine (100%), Bovine (93%), Equine (100%), Sheep (100%), Rabbit (87%), Canine (100%), Chicken (100%), Xenopus (87%)
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Protein interacting with C kinase 1 (PICK1) is a PDZ-domain containing protein that is located in the perinuclear region and is phosphorylated in response to PKC activation. PKC , which is essential for the regulation of proliferation and differentiation in numerous cell types, contains within its catalytic region a PDZ-binding domain that is absent from other PKC isoforms. Mutation of the PICK1 PDZ domain inhibits the binding of PICK1 to PKC . PICK1 also interacts with the C-terminus of a-amino-3-hydroxy-5-methyl- isoxazole-4-propionic acid (AMPA) receptor, a neurotransmitter receptor located at excitatory synapses, suggesting that PICK1 plays a role in the modulation of synaptic transmission by targeting and anchoring AMPA to specific synapses. Applications: Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight. Immunohistochemistry: 1:50-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice