PDGFB

Size

£665.00

SKU:: 526801
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to within the N-terminal of human PDGFB.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: PDGF-BB is a cystine-linked homodimer PDGF family member with key roles in development, cell proliferation, cell survival, and angiogenesis. PDGF-BB is expressed by vascular endothelium, megakaryocytes and Leydig cells. PDGF-BB targets pericytes, fibroblasts, monocytes and other cell types (1-3). PDGF induces fibroblast growth and migration (3) and is a chemoattractant for monocytes and granulocytes. Precursor PDGF-BB is cleaved intracellularly to generate a form that contains a C-terminal stretch that serves to retain PDGF-BB in the extracellular matrix. In a second cleavage event, the C-terminal stretch is removed extracellularly to generate mature PDGF-BB. PDGF-BB binds to PDGFR beta and induces receptor dimerization. Signaling is through the PI3K/Akt, JNK, and PLCγ pathways. PDGF-BB may have a role in some cancer types. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight. Immunohistochemistry: 1:50-1:200 Immunofluorescence (IC): 1:100-1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice