524347
IL1A
Size
£665.00
- SKU:
- 524347
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to human IL1A. Species sequence homology: Porcine (100%), Bovine (90%), Equine (90%), Sheep (90%), Rabbit (90%), Canine (100%)
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- IL-1alpha is a pro-inflammatory cytokine produced by activated monocytes, lymphocytes and epithelial cells (1). IL-1alpha is synthesized as an active precursor protein that appears to be cleaved by cytosolic proteases into its mature form (1,2). Often, precursor and mature forms of IL-1alpha are primarily retained intracellularly rather than constitutively secreted. (1,2). Signaling by IL-1alpha involves IL-1alpha binding to an IL-1 accessory protein (IL-1-AcP) and then the complex binds to IL-1RI (1,2). Signaling is through activation of MAP kinase and NFkappaB pathways (1,2). IL-1alpha also binds to an IL-RII that lacks an intracellular signaling domain and thereby serves as a high affinity decoy receptor. Inhibition of IL-1alpha activity is through IL-1R antagonist (IL-1Ra) that binds IL-1R1 but does not signal. IL-1alpha has been shown to be a key mediator of virus-induced inflammatory responses in mice (3). Applications: Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight. Immunohistochemistry: 1:50-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice