IKBKE

Size

£685.00

SKU:: 524277
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified recombinant fragment corresponding to human IKBKE expressed in E. coli.
Format:: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon.The transcription factor NFkappaB is retained in the cytoplasm in an inactive form by the inhibitory protein IkappaB. Activation of NFkappaB requires that IkappaB be phosphorylated on specific serine residues, which results in targeted degra-dation of IkappaB. IkappaB kinase alpha(IKKalpha), previously designated CHUK, interacts with IkappaB-alphaand specifically phosphorylates IkappaB-alpha on the sites that trigger its degradation, serines 32 and 36. The functional IKK complex contains three subunits, IKKalpha, IKKbetaand IKKgamma(also designated NEMO), and each appear to make essential contributions to IkappaB phosphorylation. IKK-i is a serine/threonine kinase that shares homology with IKKalpha and IKKbeta. IKK-i is pri-marily expressed in immune cells and is induced by lipopolysaccharide and by proinflammatory cytokines including TNFalpha, IL-1 and IL-6. Overexpression of IKK-i was shown to result in phosphorylation of IkappaB alpha on Ser32 and Ser36, and in NFkappaB activation, suggesting that IKK-i may act as an IκB kinase in the immune system. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilution: ELISA: 1:10,000 Western Blot: 1:500-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice