523687
GSTT1
Size
£665.00
- SKU:
- 523687
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to human GSTT1.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- The GST superfamily is made up of several subfamilies. Glutathione S-transferase P (GSTP1) belongs to the Pi subfamily and is involved in the conjugation of reduced glutathione to a variety of endogenous and exogenous hydrophobic electrophiles. Glutathione S-transferase Mu 1 (GSTM1) is a cytoplasmic liver protein belonging to the Mu family and has the same basic functions as GSTP1-1. Glutathione S-transferase theta 1 (GSTT1), a cytoplasmic homodimer belonging to the Theta family, is expressed in erythrocytes. It is active in the reduced glutathione conjugation and also displays glutathione peroxidase activity with cumene hydroperoxide. Applications: Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight. Immunohistochemistry: 1:50-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice