523376
GNA11
Size
£665.00
- SKU:
- 523376
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to human GNA11. Species sequence homology: Bovine (100%), Equine (100%), Sheep (100%), Canine (100%), Chicken (100%), Xenopus (100%)
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Heterotrimeric guanine nucleotide-binding proteins (G proteins) consist of alpha, beta and gamma subunits and mediate the effects of hormones, neurotransmitters, chemokines and sensory stimuli. To date, over 20 known G alpha subunits have been classified into four families, G alpha(s), G alpha(i/o), G alpha(q) and G alpha(12), based on structural and functional similarities (1,2). Phosphorylation of Tyr356 of G alpha(q)/ G alpha(11) is essential for activation of the G protein, since phenylalanine substitution for Tyr356 changes the interaction of G alpha with receptors and abolishes ligand-induced IP3 formation (3). Applications: Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight. Immunohistochemistry: 1:50-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice