ERK1/2

Size

£665.00

SKU:: 522764
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to human ERK1/2.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: ERK1 p42 MAP kinase plays a critical role in the regulation of cell growth and differentiation. Activated by a wide variety of extracellular signals including growth and neurotrophic factors, cytokines, hormones and neurotransmitters. ERK2 p44 MAP kinase plays a critical role in the regulation of cell growth and differentiation. Acts as an integration point for multiple biochemical signals, and is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunohistochemistry: 1:100-1:500 Immunofluorescence: 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice