CTNND1

Size

£665.00

SKU:: 521926
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to human CTNND1.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Catenin delta-1 (p120 catenin) has an N-terminal coiled-coil domain followed by a regulatory domain containing multiple phosphorylation sites and a central Armadillo repeat domain of ten linked 4aa repeats. The C-terminal tail has no known function (1). Catenin delta-1 fulfills critical roles in the regulation of cell-cell adhesion as it regulates E- cadherin turnover at the cell surface to determine the level of E-cadherin available for cell-cell adhesion (2). Catenin delta-1 has both positive and negative effects on cadherin-mediated adhesion (3). Actin dynamics are also regulated by catenin delta-1, which modulates RhoA, Rac, and cdc42 proteins (1). Analogous to beta-catenin, catenin delta-1 translocates to the nucleus, although its role at this location is unclear. Many studies show that catenin delta-1 is expressed irregularly or is absent in various types of tumor cells, suggesting that catenin delta-1 may function as a tumor suppressor (4). Phosphorylation of Tyr904 on Catenin-delta-1 was identified at Cell Signaling Technology (CST) using PhosphoScan, a CST's LC-MS/MS platform for phosphorylation site discovery (5). Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight. Immunohistochemistry: 1:50-1:200 Immunofluorescence (IF-IC): 1:100-1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice