Acrosin, Recombinant, Meleagris Gallopavo, aa20-346, His-Tag, Myc-Tag (ACR)

Size

£633.00

SKU:: 517787
Molecular Weight:: 43.1
Purity:: ≥85% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Extra Details:: Serine protease of trypsin-like cleavage specificity. Synthesized in a zymogen form, proacrosin and stored in the acrosome. Source: Recombinant protein corresponding to aa20-346 of Meleagris Gallopavo Acrosin, fused to 10xHis-Tag at N-terminal and Myc-Tag at C-terminal, expressed in E. coli. Molecular Weight: ~43.1kD Amino Acid Sequence: FSGGCDTCGLRPVAYHYGGMRVVGGTEALHGSWPWIVSIQNPRFAGTGHMCGGSLITPQWVLSAAHCFGRPNYILQSRVVIGANDLTQLGQEVEVRSIRRAILHEYFNNKTMINDIALLELDRPVHCSYYIQLACVPDPSLRVSELTDCYVSGWGHMGMRSAAPTQTAEVLQEAKVHLLDLNLCNSSHWYDGVLHSHNLCAGYPQGGIDTCQGDSGGPLMCRDSSADYFWLVGVTSWGRGCGRAFRPGIYTSTQHFYNWILLQVRAAAHPTSRTWSHYMSTSSYHHGPNAVPTQPSVSDSCPFPAQKLREFFTGVQNLLQSLWGSKA Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.