Tubulin, beta

Size

£840.00

SKU:: 516786
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human MBP (microtubule-binding protein).
Clone:: S11B (recomb)
Format:: Supplied as a liquid in PBS, 0.02% Proclin 300.
Extra Details:: Recombinant monoclonal antibody to Beta-Tubulin. Manufactured using Recombinant Platform with variable regions (i.e. specificity) from the scFv S11B. Microtubules are constituent parts of the mitotic apparatus, cilia, flagella and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000kD. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. For example, expression of tubulin in adipose tissue is very low and therefore beta Tubulin should not be used as loading control for these tissues. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.