Notch 1

Size

£840.00

SKU:: 516754
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: NRR of mouse Notch1.
Clone:: E6 (recomb)
Format:: Supplied as a liquid in PBS, 0.02% Proclin 300.
Extra Details:: Recombinant monoclonal antibody to Notch 1. Manufactured using Recombinant Platform with variable regions (i.e. specificity) from the phage display derived scfv E6. The Notch signaling pathway, first identified in Drosophila, regulates the generation of differentiated cells from a population of undifferentiated progenitors. The Notch1 protein is a single pass transmembrane receptor activated through the Delta (Dl) and Serrate (Ser) ligands in Drosophila (and their mammalian counterparts Delta-like-3 and Jagged 1 and 2). Activation results in the proteolytic cleavage of the cytoplasmic portion of Notch (120kD) which localizes to the nucleus to interact with DNA binding proteins (RB-Jk/KBF2/CBF1). The interaction of Notch with these proteins converts them from transcriptional repressors to activators, thus affecting the expression of a variety of target genes. Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice