516476
CD80 (APC)
Size
£607.00
- SKU:
- 516476
- Application:
- Flow Cytometry
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Hamster
- Immunogen:
- CHO cell line transfected with mouse B7 (CD80). Species Sequence Homology: canine
- Clone:
- 16-10A1
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
- Extra Details:
- CD80 is a 60kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: ≤1ug/10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Excitation Laser: Red (633 nm) Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice