Centromere Protein B (CENPB), Recombinant, His-Tag

Size

£1,445.00

SKU:: 516119
Molecular Weight:: 80
Purity:: ≥90% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Hepes-Sodium Hydroxide, pH 7.5, 0.15M sodium chloride, 0.02% sodium dodecylsulphate, 0.1mM PMSF.
Extra Details:: Centromere proteins are a group of proteins which form and/or mediate the function of centromeres, the central structures of chromosomes to which spindle fibers/microtubuli attach and pull the chromosomes apart in cell division. Currently, 9 centromere proteins are known and designated CENP-A to CENP-I. Most of the centromere proteins are targets of autoantibodies, the anti-centromere antibodies. CENP-B is the most important of the centromeric autoantigens: it has a molecular weight of approx. 66kD and plays an important role in the formation of the centromeric chromatin. CENP-B antibodies are present in the sera of up to 80% of patients with CREST syndrome. Recombinant protein corresponding to Centromere Protein B (CENPB), fused to His-Tag, expressed in insect cells. Molecular Weight: ~80kD Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice