ERK1/2 (ERK, ERK 1, ERK1, ERT2, HS44KDAP, HUMKER1A, Insulin Stimulated MAP2 Kinase, MAP Kinase 1, MAP Kinase 3, MAP Kinase Isoform p44, MAPK 1, MAPK 3, MAPK3, p44 ERK1, p44 MAPK, P44ERK1, P44MAPK, PRKM3)

Size

£570.00

SKU:: 509221
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Mitogen-activated protein kinase 3.
Clone:: 19D168
Format:: Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Extra Details:: ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to insulin and NGF. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:5000 Immunoprecipitation: 1:1000-1:10,000 Immunohistochemistry: 1:100-1:400 Immunofluorescence: 1:20-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.