Hepatitis B Virus Surface Antigen

Size

£659.00

SKU:: 506284
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified native hepatitis B surface antigen (ad+ay)
Clone:: 1838
Format:: Supplied as a liquid in in PBS, pH 7.4, 0.09% sodium azide.
Extra Details:: Hepatitis B virus (HBV) is a small, partially double-stranded DNA virus that belongs to the genus Orthohepadnaviruses of the Hepadnaviridae family of viruses. Currently, eight genotypes of HBV are recognized, designated A-H, and four subtypes (adw, adr, ayw and ayr) each having a distinct geographical distribution. HBV is a retrovirus that replicates by reverse transcription of an RNA intermediate. HBV encodes seven proteins, recognized as preCore, core, pol, X (HBx), and envelope proteins L, M and S (Liang ,TJ). First recognized in the 1940s as an infectious agent causing hepatitis, the HBV virus was eventually identified in the mid-1960’s when HBV particles were detected in a patient’s serum. The particles, initially referred to as Australia antigen, were subsequently found to be HBV envelope proteins L, S and M. Collectively these particles are now known as hepatitis B surface antigen (HBsAg) (Seeger, C). HBV is a bloodborne virus that is transmitted through contact with infected blood or bodily fluds. HBV infection may occur through various routes including the sharing of needles for injecting drugs, the use of inadequately sterilized medical equipment infected with HBV and the transfusion of unscreened blood and blood products. In endemic areas, perinatal transmission of HBV from mother to child is common. HBV infection causes liver disease which can vary from acute, or chronic hepatitis to cirrhosis of the liver and potentially hepatocellular carcinoma. The incubation of HBV infection can vary from 1 – 6 months. During the period of acute infection, most individuals remain asymptomatic. However, some patients develop acute illness presenting with clinical symptoms that include jaundice, nausea, vomiting, abdominal pain and extreme fatigue. Acute liver failure may occur in 1% of patients, which can be fatal. HBV infected patients may also develop chronic lifelong disease, which can progress to cirrhosis or hepatocellular carcinoma in 20-30% of adult cases (WHO). The asymptomatic nature of HBV infection, and the similarity of clinical symptoms to other types of hepatitis virus infection makes clinical diagnosis difficult. Therefore, laboratory diagnosis is undertaken using serological and molecular methods to detect HBsAg and specific IgM antibodies recognizing core antigen HbcAg. Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:20-1:200 Optimal dilutions to be determined by the researcher. Matched Pair: Capture: H1909-01C Detection: 506284 Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice