Chikungunya Virus E2 Protein

Size

£2,034.00

SKU:: 506233
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Inactivated native Chikungunya virus, strain 181/25.
Clone:: 16A12
Format:: Supplied as a liquid in in PBS, pH 7.4.
Extra Details:: Chikungunya virus is the aetiological agent of chikungunya fever. CHIKV belongs to the Alphavirus genus, and is an enveloped, single-stranded positive-sense RNA virus (Strauss & Strauss, 1994). The alphavirus genome encodes four non-structural proteins (nsP1 to nsP4) and five structural proteins (capsid, E3, E2, 6K and E1). CHIKV is transmitted to humans by Aedes mosquitoes, and disease is characterized by a rapid onset of fever, myalgia and often a rash (usually maculopapular), with chronic disease characterized by episodic, and often debilitating, polyarthralgia/polyarthritis. (Suhrbier et al., 2012). The largest epidemic of CHIKV disease ever reported began in 2004 and has since been responsible for up to 6.5 million human cases, primarily in Africa and Asia, with imported cases reported in over 40 countries. CHIKV infection is symptomatically similar to infection with Zika virus and Dengue virus, and differential diagnosis using immunoassay based testing is important in patient management. E2, the receptor binding protein, contains residues critical for immunogenicity, host range, and tissue/cell tropism (Voss et al, 2010). The E2 protein consists of three domains (A, B, and C), of which A and B have been found to contain the majority of residues that affect cell attachment and or tissue/cell tropism. Applications: Suitable for use in ELISA, Immunofluorescence and Neutralization. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice